Etichetă: population

CategoriesArticolePublicații

STR data for the AmpFlSTR Identifiler from Dobruja region (SE Romania)

Posted on 01.03.2009by Mugo

Stanciu F, Popescu OR, Stoian IM. Forensic Sci Int Genet. 2009 Mar;3(2):146-7. doi: 10.1016/j.fsigen.2008.09.009. Epub 2008 Oct 26. PMID: 19215887.

Abstract: 

Allele frequencies for 15 STR loci included in AmpFlSTR Identifiler kit (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) were determined in a sample of 569 unrelated iniduals living in the region of Dobruja (SE Romania). No deviations from Hardy–Weinberg equilibrium were observed. Genetic parameters of forensic interest were calculated and comparison with geographically nearby populations was performed.

Keywords: DNA typing, Short Tandem Repeats (STRs), AmpFlSTR Identifiler, Population

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CategoriesArticolePublicații

Comprehensive STR data for the AmpFISTR Identifiler from Transylvania (NW Romania)

Posted on 11.11.2008by Mugo

Stanciu F, Stoian IM, Popescu OR. Leg Med (Tokyo). 2009 Jan;11(1):48-50. doi: 10.1016/j.legalmed.2008.09.001. Epub 2008 Nov 11. PMID: 19004660.

Abstract:

Allele frequencies for 15 STR loci included in AmpFlSTR Identifiler kit (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) were determined in a sample of 1977 unrelated iniduals living in the region of Transylvania (NW Romania). No deviations from Hardy–Weinberg equilibrium were observed with the exception of D3S1358 and D16S539. Genetic parameters of forensic interest were calculated and comparison with geographically nearby populations was performed.

Keywords: Short tandem repeats (STRs); AmpFlSTR Identifiler; Population data; Transylvania

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CategoriesPosterePublicații

Indirect approach in finding someone’s autosomal genetic profile – a forensic tool in investigating historical cases

Posted on 03.09.2007by Mugo

Florin Stanciu, Romeo Potorac, Veronica Stoian. Poster at 5th ISABS Conference in Forensic Genetics and Molecular Anthropology. Split, Croatia, 3-7 September 2007.

Abstract:

Starting with an intuitive schema and a rich historical information background, it has been possible to determine the origins and the most probable genetic profile of a male person who lived between 1954 and 1984. In lack of some biological sample from this person, an indirect approach was the most suitable way in determining his genetic autosomal profile. For this purpose it has been analyzed his genealogical tree – eight saliva samples taken from his relatives and 9 biological micro-traces taken from the surface of fifth personal paper documents – a school certificate, a driving license, a handwritten letter, a communist party notebook and a receipt. DNA Isolated from all biological samples had been amplified using Identifiler and Yfiler kits. PCR products had been analyzed with ABI Prism 3100. For revealing the genealogical origins of the investigated male person, specific international DNA databases for Y and autosomal STR markers, such as YHRD, Ysearch, OmniPop and ENFSI WG STR Population Database, had been interrogated.

Keywords: genealogical tree, personal documents, most probable genetic profile, historical case, STR databases.

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CategoriesArticolePublicații

The allelic frequencies analysis of two protein markers and several DNA-STR markers in human populations

Posted on 13.01.2005by Mugo

Duţă-Cornescu G, Stoian V, Stanciu F, Simon-Gruiţă A, Rodewald A. Proceedings GSP 2005 – International Workshop on Genomic Signal Processing; p.143-148.

Abstract:

The genetic discipline cannot be uncoupled from bio-statistics, the tools to handle and analyze the large amount of data that are obtained from DNA, RNA and protein projects. The mathematics and bio-statistics give the genetic researcher the possibility to understand the genetic data, to interpret and to obtain maximum results from minimum data. The human race is characterized by variability, by polymorphisms. A polymorphism is a mendelian character which is present in at least two phenotypes in population, from which none is rare (i.e. none appears with a frequency lower then 1%-2%). At the beginning the protein polymorphisms were used in population genetic studies, but there relatively low variability and the need of large amounts of biologic material made them hard to use in large scale genetic analysis. In 1978 the first DNA polymorphism was discovered (the B – globin gene). After that a cascade of types of DNA markers came, RFLP (Restriction Fragment Length Polymorphism), VNTR (Variable Number Tandem Repeats) and STR (Short Tandem Repeats), and the analysis of the genetic structure of human populations became easy, the challenging part for a biologist being now the interpretation, the formulation of an equation in which all the data to be present This was possible by creating a mathematical model, a representation of the biological processes, in which both observed laboratory data and expected data are described in quantitative way.
In this paper, the authors present a large picture of how mathematics and statistic help a researcher to give a meaning of his genetic observation. We used the polymorphism of two proteins (Haptoglobin and Transferrin) and two STR – DNA markers to make a population survey and to compare the genetic structure of Romanian population with other European and non-European population. The data were interpreted in statistical method, under Hardy-Weinberg condition, using X tests. Also, using several other STR DNA markers and math methods we exemplify bow linkage analysis can be a powerful tool for prenatal diagnostic of different genetic disorders and for finding new genes.

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