Identifiler

Allele frequencies of 15 STR loci in Moldavia region (NE Romania)

Posted on 08.04.2009by Mugo

Stanciu F, Popescu OR, Stoian IM. Forensic Sci Int Genet. 2009 Dec;4(1):e39-40. doi: 10.1016/j.fsigen.2009.02.008. Epub 2009 Apr 8. PMID: 19948322.

Abstract:

Allele frequencies for 15 STR loci included in AmpFlSTR Identifiler kit (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) were determined in a sample of 1321 unrelated iniduals living in the region of Moldavia (NE Romania). No deviations from Hardy–Weinberg equilibrium were observed (only after applying the Bonferroni correction in the cases of D2S1338). Genetic parameters of forensic interest were calculated and comparison with geographically nearby populations was performed.

Keywords:DNA typing, Short tandem repeats (STRs), AmpFlSTR Identifiler, Population data, Moldavia

Download

Articole Publicații

STR data for the AmpFlSTR Identifiler from Dobruja region (SE Romania)

Posted on 01.03.2009by Mugo

Stanciu F, Popescu OR, Stoian IM. Forensic Sci Int Genet. 2009 Mar;3(2):146-7. doi: 10.1016/j.fsigen.2008.09.009. Epub 2008 Oct 26. PMID: 19215887.

Abstract:

Allele frequencies for 15 STR loci included in AmpFlSTR Identifiler kit (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) were determined in a sample of 569 unrelated iniduals living in the region of Dobruja (SE Romania). No deviations from Hardy–Weinberg equilibrium were observed. Genetic parameters of forensic interest were calculated and comparison with geographically nearby populations was performed.

Keywords: DNA typing, Short Tandem Repeats (STRs), AmpFlSTR Identifiler, Population

Download

Articole Publicații

Comprehensive STR data for the AmpFISTR Identifiler from Transylvania (NW Romania)

Posted on 11.11.2008by Mugo

Stanciu F, Stoian IM, Popescu OR. Leg Med (Tokyo). 2009 Jan;11(1):48-50. doi: 10.1016/j.legalmed.2008.09.001. Epub 2008 Nov 11. PMID: 19004660.

Abstract:

Allele frequencies for 15 STR loci included in AmpFlSTR Identifiler kit (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) were determined in a sample of 1977 unrelated iniduals living in the region of Transylvania (NW Romania). No deviations from Hardy–Weinberg equilibrium were observed with the exception of D3S1358 and D16S539. Genetic parameters of forensic interest were calculated and comparison with geographically nearby populations was performed.

Keywords: Short tandem repeats (STRs); AmpFlSTR Identifiler; Population data; Transylvania

Download

Postere Publicații

Valorificarea reziduurilor biologice obținute în urma analizelor medicale pentru studii de Genetică Forensică

Posted on 18.09.2008by Mugo

Stanciu F, Sevastre O, Tinischi M, Stoian V. Poster la 4th Medical Genetic Romanian Association Conference, 18-20 Septembrie 2008 Craiova, România.

Abstract:

În situații deosebite în care recoltarea de probe biologice de referință este imposibilă prin metodele clasice, analizele medicale efectuate în laboratoarele de screening, respectiv reziduurile biologice obținute în urma efectuării acestora, pot furniza date genetice valoroase cu aplicabilitate în genetica forensică. În acest sens am evaluat o serie de reziduuri in componența cărora pot exista celule nucleate pretabile genotipării, urmărind factori precum timpul și condițiile de păstrare ale probelor biologice în laboratoarele medicale, degradarea ADN-ului, prezența contaminării, rata succesului calculata la numărul de loci amplificaţi, etc.. Am efectuat izolarea totală a ADN-ului folosind o forma adaptata a protocolului AGOWA mag DNA Isolation Kit pentru platforma automată de manipulare a lichidelor Freedom EVO 150. Din extractele de ADN obținute au fost amplificați 16 markeri STR folosind AmpFlSTR Identifiler PCR Amplification Kit. Ampliconii au fost analizați prin electroforeza capilară folosind analizorul ABIPrism 3100. Rezultatele obținute au fost interpretate folosind GeneScan și Genotyper Software conform standardelor specifice analizelor de genetica forensică.

Download

Postere Publicații

Indirect approach in finding someone’s autosomal genetic profile – a forensic tool in investigating historical cases

Posted on 03.09.2007by Mugo

Florin Stanciu, Romeo Potorac, Veronica Stoian. Poster at 5th ISABS Conference in Forensic Genetics and Molecular Anthropology. Split, Croatia, 3-7 September 2007.

Abstract:

Starting with an intuitive schema and a rich historical information background, it has been possible to determine the origins and the most probable genetic profile of a male person who lived between 1954 and 1984. In lack of some biological sample from this person, an indirect approach was the most suitable way in determining his genetic autosomal profile. For this purpose it has been analyzed his genealogical tree – eight saliva samples taken from his relatives and 9 biological micro-traces taken from the surface of fifth personal paper documents – a school certificate, a driving license, a handwritten letter, a communist party notebook and a receipt. DNA Isolated from all biological samples had been amplified using Identifiler and Yfiler kits. PCR products had been analyzed with ABI Prism 3100. For revealing the genealogical origins of the investigated male person, specific international DNA databases for Y and autosomal STR markers, such as YHRD, Ysearch, OmniPop and ENFSI WG STR Population Database, had been interrogated.

Keywords: genealogical tree, personal documents, most probable genetic profile, historical case, STR databases.

Download