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Examinarea și interpretarea microurmelor prezente pe obiecte cu valoare istorică, prin genotipare judiciară

Stanciu F. Revista de Criminologie, de Criminalistica și de Penologie, 2007;2-3:71-81.

Abstract:

Persistența materialului genetic, în timp, este o certitudine susținută de multiplele analize genetice efectuate, în ultimele două decenii, asupra unor probe biologice vechi și foarte vechi, permițând cercetătorilor care studiază diferite aspecte ale istoriei, noi tehnici de lucru (derivate din domenii precum genetica, biochimia și criminalistica), în stabilirea realității istorice.

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Identificarea suspecților de acte teroriste și a colaboratorilor acestora, pe baza determinării indirecte a profilelor lor genetice

Stanciu F. Criminalistica. 2006;(6):12-15.

Abstract

Persistența materialului genetic în timp este o certitudine susținută de multiplele analize genetice efectuate în ultimele doua decenii asupra unor probe biologice vechi și foarte vechi, permițând cercetătorilor care studiază diferite aspecte ale istoriei noi tehnici de lucru (derivate din domenii precum genetica, biochimia și criminalistica), în stabilirea realității istorice.
Bazându-se pe cazuri consacrate în domeniul genetice judiciare istorice dar și pe propria experiență, autorul își propune prin articolul de fata sa reliefeze succint aspectele particulare legate de analiza microurmelor de natura biologica dispuse pe obiecte cu valoare istorică și aportul potențial al acestora în clarificare disputelor din jurul unor evenimente sau personalități istorice

Cuvinte cheie: microurme de natura biologica, markeri ADN mitocondriali, markeri ADN nucleari, Charles Robert Darwin, Jack Spintecătorul

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Comparative study of total DNA isolation methods from different types of biological samples

Duţă-Cornescu G, Stanciu F, Neagoe I, Stoian V. Romanian Biotechnological Letters, 2005;10(1):2011-2018.

Abstract:

Any genetic analysis it’s starts with the genetic material extraction. On the quality and quantity of the extracted DNA or RNA depends the success of any further research. The development of new molecular techniques have made possible for us to analyse any type of biological sample, from an insect embedded in residues, to fossils and to forensic samples.
In this article the authors are presenting a comparative study of the most used DNA extraction methods and there own experimental modifications, using as starting material a wide range of biological samples. The purpose of this study is to create the best DNA extraction method adapted to the particularities of our samples and our laboratory. These methods will be used to obtain a high quality DNA, suitable for our further experiments

Keywords: extraction techniques, DNA, tumour, ancient human bones, blood

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The allelic frequencies analysis of two protein markers and several DNA-STR markers in human populations

Duţă-Cornescu G, Stoian V, Stanciu F, Simon-Gruiţă A, Rodewald A. Proceedings GSP 2005 – International Workshop on Genomic Signal Processing; p.143-148.

Abstract:

The genetic discipline cannot be uncoupled from bio-statistics, the tools to handle and analyze the large amount of data that are obtained from DNA, RNA and protein projects. The mathematics and bio-statistics give the genetic researcher the possibility to understand the genetic data, to interpret and to obtain maximum results from minimum data. The human race is characterized by variability, by polymorphisms. A polymorphism is a mendelian character which is present in at least two phenotypes in population, from which none is rare (i.e. none appears with a frequency lower then 1%-2%). At the beginning the protein polymorphisms were used in population genetic studies, but there relatively low variability and the need of large amounts of biologic material made them hard to use in large scale genetic analysis. In 1978 the first DNA polymorphism was discovered (the B – globin gene). After that a cascade of types of DNA markers came, RFLP (Restriction Fragment Length Polymorphism), VNTR (Variable Number Tandem Repeats) and STR (Short Tandem Repeats), and the analysis of the genetic structure of human populations became easy, the challenging part for a biologist being now the interpretation, the formulation of an equation in which all the data to be present This was possible by creating a mathematical model, a representation of the biological processes, in which both observed laboratory data and expected data are described in quantitative way.
In this paper, the authors present a large picture of how mathematics and statistic help a researcher to give a meaning of his genetic observation. We used the polymorphism of two proteins (Haptoglobin and Transferrin) and two STR – DNA markers to make a population survey and to compare the genetic structure of Romanian population with other European and non-European population. The data were interpreted in statistical method, under Hardy-Weinberg condition, using X tests. Also, using several other STR DNA markers and math methods we exemplify bow linkage analysis can be a powerful tool for prenatal diagnostic of different genetic disorders and for finding new genes.

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