CategoriesArticolePublicații

Genetic parameters and allele frequencies of five new European Standard Set STR loci (D10S1248, D22S1045, D2S441, D1S1656, D12S391) in the population of Romania

Stanciu F, Vladu S, Cutar V, Cocioaba D, Iancu F, Cotolea A, Stoian IM. Croat Med J. 2013 Jun;54(3):232-7. doi: 10.3325/cmj.2013.54.232. PMID: 23771753; PMCID: PMC3692331.

Aim: To establish allele frequencies and genetic parameters for 5 new European Standard Set short tandem repeat (STR) loci in the population of Romania and to compare them with those in other populations.

Methods: DNA was isolated using QIAamp 96 DNA Swab BioRobot Kit and Chelex 100 methods. Polymerase chain reaction amplification was done using Investigator ESSplexPlus Kit (D1S1656, D2S441, D2S1338, D3S1358, D8S1179, D10S1248, D12S391, D16S539, D18S51, D19S433, D21S11, D22S1045, FGA, TH01, and vWA). For DNA typing, Applied Biosystems 3500/3500xL Genetic Analyzer was used. Statistical analysis was done using Powerstats, GDA, and Arlequin software.

Results: Power of discrimination and polymorphism information content was highest for two new ESS loci, D1S1656 and D12S391. Comparison of allele frequencies for 5 new ESS loci in Romanian population with previously published population data showed significant differences for all compared populations, with the exception of Hungary. Geographically more distant populations, such as Spain, Sweden, United Kingdom, Germany, and Portugal differed more than closer populations.

Conclusion: New ESS STR loci are very useful for the analysis of forensic samples (persons or traces) due to their characteristics (shortness and high polymorphism). In comparisons with other common STR markers, they have a higher power of discrimination and also higher polymorphism information content, and could be used in any national DNA database.

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CategoriesPostere

Building a minimalist DNA database management system using Pruem tools

Stanciu F. Poster at 10th International Symposium on Forensic Sciences, 27-30 September 2011, Bratislava, Slovakia.

Abstract:

When it comes of DNA data exchange base on 2008/615/JHA and 2008/616/JHA Council Decisions, each EU country is free of choosing the implementation means. Romania has some experience in implementing three different types of IT solutions: a bought one – Dimensions 2.0, an in-house built solution – Pruem tools and a freeware solution – CODIS 7.0, each of them with their specific advantages and disadvantages. Starting with the Pruem DNA data exchange infrastructure and base on Pruem tools any DNA database laboratory, which has some Pruem DNA data exchange experience, can build from scratch a simple DNA database management system for laboratory internal DNA profiles comparison purposes, with a minimum of human and IT resources. This paper presents a review of the Romanian National DNA Database Laboratory efforts in building a minimalist DNA database management system using the most common and freely available Pruem tools: Communication component (Germany), Match engine (Austria) and Graphical user interface (Netherlands).

Keywords: Pruem DNA data exchange, Pruem tools, DNA database management system, Romanian National DNA Database Laboratory.

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CategoriesArticolePublicații

Allele frequencies of 15 STR loci in Moldavia region (NE Romania)

Stanciu F, Popescu OR, Stoian IM. Forensic Sci Int Genet. 2009 Dec;4(1):e39-40. doi: 10.1016/j.fsigen.2009.02.008. Epub 2009 Apr 8. PMID: 19948322.

Abstract:

Allele frequencies for 15 STR loci included in AmpFlSTR Identifiler kit (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) were determined in a sample of 1321 unrelated iniduals living in the region of Moldavia (NE Romania). No deviations from Hardy–Weinberg equilibrium were observed (only after applying the Bonferroni correction in the cases of D2S1338). Genetic parameters of forensic interest were calculated and comparison with geographically nearby populations was performed.

Keywords:DNA typing, Short tandem repeats (STRs), AmpFlSTR Identifiler, Population data, Moldavia

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CategoriesArticolePublicații

Automated DNA isolation for databasing purposes

Stanciu F, Stoian IM, Popescu OR. Rom J Leg Med 2009;17(1):51–58

Abstract:

Nowadays, automation is crucial in databasing laboratories especially for countries which are relative new in the field and must quickly reach the minimum standards of consecrated databasing laboratories from Europe and USA. In our efforts to supply genetic profiles for Romanian National DNA Database, we evaluated the Freedom Evo 150 liquid handling platform with AGOWA sep9600 magnetic separator in different running conditions and workflows, for reaching the most efficient ones. We compared the results with those obtained from manual DNA isolation with Chelex resin and paramagnetic particles. For this we used a total of 6246 biological reference samples (5220 – automated processed and 1026 – manually processed), amplified using AmpFlstr Identifiler Kit and analyzed by capillary electrophoresis using ABI Prism 3100 Genetic Analyzer.

Keywords: automation, databasing laboratory, liquid handling platform, DNA magnetic separator.

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CategoriesArticolePublicații

STR data for the AmpFlSTR Identifiler from Dobruja region (SE Romania)

Stanciu F, Popescu OR, Stoian IM. Forensic Sci Int Genet. 2009 Mar;3(2):146-7. doi: 10.1016/j.fsigen.2008.09.009. Epub 2008 Oct 26. PMID: 19215887.

Abstract: 

Allele frequencies for 15 STR loci included in AmpFlSTR Identifiler kit (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) were determined in a sample of 569 unrelated iniduals living in the region of Dobruja (SE Romania). No deviations from Hardy–Weinberg equilibrium were observed. Genetic parameters of forensic interest were calculated and comparison with geographically nearby populations was performed.

Keywords: DNA typing, Short Tandem Repeats (STRs), AmpFlSTR Identifiler, Population

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