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Certificatul de Urbanism

Conform legislației în vigoare Certificatul de Urbanism este actul de informare prin care administrația publică locală (…), în conformitate cu planurile urbanistice și ale regulamentelor aferente acestora ori ale planurilor de amenajare a teritoriului, după caz, avizate și aprobate potrivit legii, fac cunoscute solicitantului elementele privind regimul juridic, economic și tehnic al terenurilor și construcțiilor existente la data solicitării și stabilesc cerințele urbanistice care urmează să fie îndeplinite în funcție de specificul amplasamentului, precum și lista cuprinzând avizele și acordurile legale, necesare în vederea autorizării (Legea 50/1991).

CategoriesArticolePublicații

Genetic parameters and allele frequencies of five new European Standard Set STR loci (D10S1248, D22S1045, D2S441, D1S1656, D12S391) in the population of Romania

Stanciu F, Vladu S, Cutar V, Cocioaba D, Iancu F, Cotolea A, Stoian IM. Croat Med J. 2013 Jun;54(3):232-7. doi: 10.3325/cmj.2013.54.232. PMID: 23771753; PMCID: PMC3692331.

Aim: To establish allele frequencies and genetic parameters for 5 new European Standard Set short tandem repeat (STR) loci in the population of Romania and to compare them with those in other populations.

Methods: DNA was isolated using QIAamp 96 DNA Swab BioRobot Kit and Chelex 100 methods. Polymerase chain reaction amplification was done using Investigator ESSplexPlus Kit (D1S1656, D2S441, D2S1338, D3S1358, D8S1179, D10S1248, D12S391, D16S539, D18S51, D19S433, D21S11, D22S1045, FGA, TH01, and vWA). For DNA typing, Applied Biosystems 3500/3500xL Genetic Analyzer was used. Statistical analysis was done using Powerstats, GDA, and Arlequin software.

Results: Power of discrimination and polymorphism information content was highest for two new ESS loci, D1S1656 and D12S391. Comparison of allele frequencies for 5 new ESS loci in Romanian population with previously published population data showed significant differences for all compared populations, with the exception of Hungary. Geographically more distant populations, such as Spain, Sweden, United Kingdom, Germany, and Portugal differed more than closer populations.

Conclusion: New ESS STR loci are very useful for the analysis of forensic samples (persons or traces) due to their characteristics (shortness and high polymorphism). In comparisons with other common STR markers, they have a higher power of discrimination and also higher polymorphism information content, and could be used in any national DNA database.

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CategoriesArticolePublicații

Population Data for 15 Short Tandem Repeat Loci from Wallachia Region, South Romania

Stanciu F, Stoian IM, Popescu OR. Croat Med J. 2009 Jun;50(3):321-5. doi: 10.3325/cmj.2009.50.321. PMID: 19480027; PMCID: PMC2702745.

Aim: To determine allele frequencies’ distribution for 15 short tandem repeat (STR) loci in a population sample of 1910 unrelated iniduals from the region of Wallachia, South Romania.

Methods: DNA was isolated using Chelex 100 method and an adapted version of AGOWA mag DNA Isolation Kit Sputum. Polymerase chain reaction amplification was done using AmpFlSTR Identifiler kit (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA). For DNA typing, ABI PRISM 3100 Genetic Analyzer was used. Genetic parameters of forensic interest were calculated and comparisons with geographically close populations were performed.

Results: With the exception of vWA locus (P=0.001), no other significant deviations from Hardy-Weinberg expectations were found. Single locus comparisons with data on geographically close populations showed significant differences between the population of Wallachia and the population of Bucharest area, Greece, Turkey, Italy, Hungary, Belarus, and Poland, but no differences were found from the population from Croatia and Serbia.

Conclusion: According to 15 analyzed STR loci, the population of Wallachia region was found to be genetically more similar to Slavic populations of Croatia and Serbia than to other surrounding populations.

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CategoriesPosterePublicații

Comparative study of soils from several Romanian South-East regions and their influence on DNA degradation in tooth samples, from different periods of time

Stoian MG, Galan E, Stanciu F, Ciofu V. Poster at Forensica 2008, Praque, Czech Republic, 25-26 April 2008.

Abstract:

The degradation process of tooth and bone tissues and also of the DNA from them is a very complex one, determined by many concurrent factors which can be classified in three main categories – biological, physical and chemical factors. This paper presents our research regarding morphological aspects of soils and the influence of chemical composition on DNA degradation through fossilization process. In order to achieve our goals, we collected 27 soil samples from different archaeological sites, from various depths and different archeologically periods of time – Neolithic, The Bronze Age, The First Iron Age, The Romano-Byzantine Age, VIII-X century. We performed pH determination, calcination losses, SEM/EDS analysis of soil samples and we correlated our results with the DNA degradation index obtained from fossilized tooth samples collected from the same locations as soils. The results obtained from this analysis represent a small part of our efforts in reaching out the main objectives: a) establishing the identification and differentiation criteria for forensic soils; b) establishing which types of soil better preserves the human DNA from teeth and bones for further genetic analyses.

Keywords: Soil, Fossilization, Teeth, DNA degradation index

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CategoriesPosterePublicații

Evaluation of Freedom Evo 150 with AGOWA sep9600 for databasing purposes

Stanciu F, Stoian IM, Popescu OR. Poster at Forensica 2008, Praque, Czech Republic, 25-26 April 2008.

Abstract:

Automation is crucial nowadays in databasing laboratories especially in those countries which are relative new in the field and must quickly reach the minimum standards of consecrated databasing laboratories from Europe and USA. In our efforts to build Romanian National DNA Database we evaluated the Freedom Evo 150 Liquid Handling Platform with Agowa 9600 Magnetic Separator in different running conditions and flux implementations, for reaching the most efficient ones. We compared the results with those obtained from manual extraction methods such as magnetic DNA extraction and Chelex DNA extraction. For this we used a total of 6246 biological reference samples (5220 – automated DNA extraction and 1026 – manually DNA extraction), which we amplified using AmpFlstr Identifiler Kit. The obtained PCR products had been analyzed by capillary electrophoresis using ABI Prism 3100 Genetic Analyzer.

Keywords: automation, databasing laboratory, liquid handling platform, DNA magnetic separator

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