Comparative study of several tooth samples from different archaeological sites and the implications of fossilization in preservation of DNA overtime

Florin Stanciu, Elena Galan, Maria G. Stoian, Georgeta Andreescu, Andrei D. Soficaru, Nicolae Miritoiu. Poster at 5th ISABS Conference in and Molecular Anthropology. Split, Croatia, 3-7 September 2007.


Several human tooth samples from Neolithic Period, Bronze Age, Iron Period and VIII-X Century Period, collected from different archaeological sites (belonging to the same geographic area – South-East of Romania) had been analyzed. Tooth extracts had been obtained using a GuSCN isolation protocol. Based on spectophotometric readings on extracts, it was possible to determine a degradation index using tooth samples from our days as etalon for degradation occurred in the steps of isolation protocol. The results were correlated to elemental composition of tooth as well as their calcinations residues, in order to relieve the effect of fossilization process on preservation. For qualitative and quantitative analysis, XRF and SEM/EDS techniques have been used.
We consider this type of knowledge being of great interest because investigations about particularities of molecular processes involved in bone and tooth conservation (or degradation) are just a few, insuffently for problems rise by this matter.

Keywords: degradation index, Fossilization, Teeth, Paleotics, Taphonomy


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