Automated DNA isolation for databasing purposes

Stanciu F, Stoian IM, Popescu OR. Rom J Leg Med 2009;17(1):51–58


Nowadays, is crucial in databasing laboratories especially for countries which are relative new in the field and must quickly reach the minimum standards of consecrated databasing laboratories from Europe and USA. In our efforts to supply tic profiles for Romanian National  Database, we evaluated the Freedom Evo 150 with AGOWA sep9600 magnetic separator in different running conditions and workflows, for reaching the most effent ones. We compared the results with those obtained from isolation with Chelex resin and paramagnetic particles. For this we used a total of 6246 biological reference samples (5220 – automated processed and 1026 – ly processed), amplified using AmpFlstr Kit and analyzed by capillary electrophoresis using ABI Prism 3100 tic Analyzer.

Keywords:, , , magnetic separator.


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