Mugo – Pagina 39

Comparative study of soils from several Romanian South-East regions and their influence on DNA degradation in tooth samples, from different periods of time

Posted on 25.04.2008by Mugo

Stoian MG, Galan E, Stanciu F, Ciofu V. Poster at Forensica 2008, Praque, Czech Republic, 25-26 April 2008.

Abstract:

The degradation process of tooth and bone tissues and also of the DNA from them is a very complex one, determined by many concurrent factors which can be classified in three main categories – biological, physical and chemical factors. This paper presents our research regarding morphological aspects of soils and the influence of chemical composition on DNA degradation through fossilization process. In order to achieve our goals, we collected 27 soil samples from different archaeological sites, from various depths and different archeologically periods of time – Neolithic, The Bronze Age, The First Iron Age, The Romano-Byzantine Age, VIII-X century. We performed pH determination, calcination losses, SEM/EDS analysis of soil samples and we correlated our results with the DNA degradation index obtained from fossilized tooth samples collected from the same locations as soils. The results obtained from this analysis represent a small part of our efforts in reaching out the main objectives: a) establishing the identification and differentiation criteria for forensic soils; b) establishing which types of soil better preserves the human DNA from teeth and bones for further genetic analyses.

Keywords: Soil, Fossilization, Teeth, DNA degradation index

Download

Postere Publicații

Evaluation of Freedom Evo 150 with AGOWA sep9600 for databasing purposes

Posted on 25.04.2008by Mugo

Stanciu F, Stoian IM, Popescu OR. Poster at Forensica 2008, Praque, Czech Republic, 25-26 April 2008.

Abstract:

Automation is crucial nowadays in databasing laboratories especially in those countries which are relative new in the field and must quickly reach the minimum standards of consecrated databasing laboratories from Europe and USA. In our efforts to build Romanian National DNA Database we evaluated the Freedom Evo 150 Liquid Handling Platform with Agowa 9600 Magnetic Separator in different running conditions and flux implementations, for reaching the most efficient ones. We compared the results with those obtained from manual extraction methods such as magnetic DNA extraction and Chelex DNA extraction. For this we used a total of 6246 biological reference samples (5220 – automated DNA extraction and 1026 – manually DNA extraction), which we amplified using AmpFlstr Identifiler Kit. The obtained PCR products had been analyzed by capillary electrophoresis using ABI Prism 3100 Genetic Analyzer.

Keywords: automation, databasing laboratory, liquid handling platform, DNA magnetic separator

Download

Postere Publicații

Indirect approach in finding someone’s autosomal genetic profile – a forensic tool in investigating historical cases

Posted on 03.09.2007by Mugo

Florin Stanciu, Romeo Potorac, Veronica Stoian. Poster at 5th ISABS Conference in Forensic Genetics and Molecular Anthropology. Split, Croatia, 3-7 September 2007.

Abstract:

Starting with an intuitive schema and a rich historical information background, it has been possible to determine the origins and the most probable genetic profile of a male person who lived between 1954 and 1984. In lack of some biological sample from this person, an indirect approach was the most suitable way in determining his genetic autosomal profile. For this purpose it has been analyzed his genealogical tree – eight saliva samples taken from his relatives and 9 biological micro-traces taken from the surface of fifth personal paper documents – a school certificate, a driving license, a handwritten letter, a communist party notebook and a receipt. DNA Isolated from all biological samples had been amplified using Identifiler and Yfiler kits. PCR products had been analyzed with ABI Prism 3100. For revealing the genealogical origins of the investigated male person, specific international DNA databases for Y and autosomal STR markers, such as YHRD, Ysearch, OmniPop and ENFSI WG STR Population Database, had been interrogated.

Keywords: genealogical tree, personal documents, most probable genetic profile, historical case, STR databases.

Download

Postere Publicații

Comparative study of several tooth samples from different archaeological sites and the implications of fossilization in preservation of DNA overtime

Posted on 03.09.2007by Mugo

Florin Stanciu, Elena Galan, Maria G. Stoian, Georgeta Andreescu, Andrei D. Soficaru, Nicolae Miritoiu. Poster at 5th ISABS Conference in Forensic Genetics and Molecular Anthropology. Split, Croatia, 3-7 September 2007.

Abstract:

Several human tooth samples from Neolithic Period, Bronze Age, Iron Period and VIII-X Century Period, collected from different archaeological sites (belonging to the same geographic area – South-East of Romania) had been analyzed. Tooth DNA extracts had been obtained using a GuSCN DNA isolation protocol. Based on spectophotometric readings on DNA extracts, it was possible to determine a DNA degradation index using tooth samples from our days as etalon for DNA degradation occurred in the steps of isolation protocol. The results were correlated to elemental composition of tooth as well as their calcinations residues, in order to relieve the effect of fossilization process on DNA preservation. For qualitative and quantitative analysis, XRF and SEM/EDS techniques have been used.
We consider this type of knowledge being of great interest because investigations about particularities of molecular processes involved in bone and tooth DNA conservation (or degradation) are just a few, insufficiently for problems rise by this matter.

Keywords: DNA degradation index, Fossilization, Teeth, Paleogenetics, Taphonomy

Download

Articole Publicații

Examinarea și interpretarea microurmelor prezente pe obiecte cu valoare istorică, prin genotipare judiciară

Posted on 20.08.2007by Mugo

Stanciu F. Revista de Criminologie, de Criminalistica și de Penologie, 2007;2-3:71-81.

Abstract:

Persistența materialului genetic, în timp, este o certitudine susținută de multiplele analize genetice efectuate, în ultimele două decenii, asupra unor probe biologice vechi și foarte vechi, permițând cercetătorilor care studiază diferite aspecte ale istoriei, noi tehnici de lucru (derivate din domenii precum genetica, biochimia și criminalistica), în stabilirea realității istorice.