A comparative study of ARMS-PCR and RFLP-PCR as methods for rapid SNP identification

Duţă-Cornescu G, Simon-Gruiţă A, Constantin N, Stanciu F, Dobre M, Banică D, Tuduce R, Cristea P, Stoian V. Romanian Biotechnological Letters, 2009;14(6):4845-4850.


Identification of single nucleotide (SNPs) is now possible by many techniques, but choosing one of these methods for a particular case represents quite a challenge, because the researcher must take into consideration many factors.
In this article the authors are trying to present a comparative study of two methods, used currently in our laboratory, for identification of SNPs : ARMS – PCR (amplification refractory mutation system) and RFLP – PCR (restriction fragment length polymorphism). The two SNPs on which we focused belong to human VDR (vitamin D receptor ) and are ApaI (a G→T base change in intron 8) and TaqI (a silent T→C base change in codon 352), named after the restriction enzymes which recognize these variations.
Since the results obtained by both methods were confirmed by direct , we concluded that method is the most adequate for detecting the alternative genotypes determined by single base mutations. The simplty of this method makes it suitable for the analysis of large number of samples, situation which is usually met in case-control and tic studies because this test is easy to use, cost – effective and have an accuracy of 99,9%

Keywords:, RFLP-PCR, , VDR , Apa I and Taq I


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